DECBI - Artigos publicados em periódicos
URI permanente para esta coleçãohttp://www.hml.repositorio.ufop.br/handle/123456789/571
Navegar
748 resultados
Resultados da Pesquisa
Item Heart rate variability : from recording precautions to applicability to physical exercise.(2022) Souza, Perciliany Martins de; Araújo, Cássia Regina Vieira; Mocaiber, Izabela; Norte, Carlos Eduardo; Becker, Lenice Kappes; Souza, Gabriela Guerra Leal deAim: This narrative review sought to address heart rate variability (HRV) based on concepts and definitions, methods of recording and processing signals, signal interpretation, influencing factors, and applicability to physical exercise. Methods: Bibliographic survey of works published between 2000 and 2022 in PubMed, Scopus, Web of Science, Scielo databases. Results: HRV is a non-invasive method for measuring the action of the autonomic nervous system in the heart and has been used as a marker of physical and mental health. HRV measurements can be performed by recording an electrocardiogram or frequency meter, which allows the extraction of several HRV parameters using linear and non-linear methods. Conclusion: HRV is a variable that can be influenced by several factors, and its role can be explained based on four theories. Physical training can be used as a marker of exercise intensity control.Item Metabolomic and elemental profiling of blood serum in bladder cancer.(2022) Ossoliński, Krzysztof; Ruman, Tomasz; Copié, Valérie; Tripet, Brian P.; Nogueira, Leonardo Brandão; Nogueira, Katiane de Oliveira Pinto Coelho; Kołodziej, Artur; Altamer, Aneta Płaza; Ossolinska, Anna; Ossolinski, Tadeusz; Nizioł, JoannaBladder cancer (BC) is one of the most frequently diagnosed types of urinary cancer. Despite advances in treatment methods, no specific biomarkers are currently in use. Targeted and untargeted profiling of metabolites and elements of human blood serum from 100 BC patients and the same number of normal controls (NCs), with external validation, was attempted using three analytical methods, i.e., nuclear magnetic resonance, gold and silver-109 nanoparticle-based laser desorption/ionization mass spectrometry (LDI-MS), and inductively coupled plasma optical emission spectrometry (ICP-OES). All results were subjected to multivariate statistical analysis. Four potential serum biomarkers of BC, namely, isobutyrate, pyroglutamate, choline, and acetate, were quantified with proton nuclear magnetic resonance, which had excellent predictive ability as judged by the area under the curve (AUC) value of 0.999. Two elements, Li and Fe, were also found to distinguish between cancer and control samples, as judged from ICP-OES data and AUC of 0.807 (in validation set). Twenty-five putatively identified compounds, mostly related to glycans and lipids, differentiated BC from NCs, as detected using LDI-MS. Five serum metabolites were found to discriminate between tumor grades and nine metabolites between tumor stages. The results from three different analytical platforms demonstrate that the identified distinct serum metabolites and metal elements have potential to be used for noninvasive detection, staging, and grading of BC.Item New insights into plant natriuretic peptide evolution : from the lysogenic conversion in Xanthomonas to the lateral transfer to the whitefly Bemisia tabaci.(2022) Patané, José Salvatore Leister; Moreira, Leandro Marcio; Teixeira, Marcus de Melo; Martins Junior, Joaquim; Setubal, João Carlos; Varani, Alessandro de MelloPlant natriuretic peptide-like (PNP) are signaling molecules related to adaptive responses to stress. The Arabidopsis thaliana PNP (AtPNP-A) is capable of modulating catalase 2 (CAT2) and rubisco activase (RCA) activity in some circumstances. Interestingly, many plant-pathogens co-opted PNP-like molecules to their benefit. For instance, the citrus pathogen Xanthomonas citri carries a PNP-like (XacPNP) that can mimic and regulate plant homeostasis, and many phytopathogenic fungi carry effectors (e.g., Ave1 and AvrLm6) that are indeed PNP-like homologs. This work investigates the PNP-like evolution across the tree of life, revealing many parallel gains and duplications in plant and fungi kingdoms. All PNP-like proteins in the final dataset are structurally similar, containing the AtPNP-A active domains modulating CAT2 activity and RCA interaction. Comparative genomics evinced that XacPNP is a lysogenic conversion factor associated with a Myoviridae-like prophage identified in many Xanthomonas species. Surprisingly, a PNP-like homolog was identified in Bemisia tabaci, an important agricultural pest, being to date the second example of lateral gene transfer (LGT) from plant to the whitefly. Moreover, the Bemisia PNP-like homolog can also be considered a potential new effector of this phloem-feeding insect. Noteworthy, the whiteflies infest many plants carrying PNP-like copies and interact with some of their bacterial and fungal pathogens, strongly suggesting complex recipient/donor traits of PNP by LGT and bringing new insights into the evolution of host-pathogen arms race across the tree of life.Item Cardiac changes in spontaneously hypertensive rats : modulation by aerobic exercise.(2023) Teles, Maria Cecília; Portes, Alexandre Martins Oliveira; Coelho, Bianca Iara Campos; Resende, Letícia Teresinha; Isoldi, Mauro CésarSystemic arterial hypertension is a multifactorial clinical condition characterized by high and sustained levels of blood pressure. For a better understanding of the pathophysiology of hypertension, studies are conducted with spontaneously hypertensive animals, which allow the investigation of physiological changes that in most cases cannot be studied in humans. In these animals, myocardial remodeling, increased pro-inflammatory markers, redox imbalance and contractile dysfunctions that lead to changes in cardiac function can be observed. However, it can be inferring that aerobic training improves cardiac function and cardiomyocyte contractility, in addition to controlling inflammation and reducing oxidative stress in cardiac muscle, despite this, the precise mechanisms by which physical exercise improves cardiovascular control are not fully understood. In this review, we provide an overview of the pathophysiological changes that affect the heart of spontaneously hypertensive animals and their modulation by aerobic exercise.Item The impact of a violent community on mental health and the benefits of a sport program for social development.(2023) Fernandes Junior, Orlando; Silva Júnior, Ruy Marra da; Ramos, Lucas Rego; Gama, Tamires M.; Lobo, Isabela; Souza, Gabriela Guerra Leal de; Sanchez, Tiago ArrudaThe aim of the present study was to evaluate the impact of a violent environment on mental health and the impact of a sport for social development (SSD) program on quality of life, mental distress symptoms, and heart rate variability (HRV). HRV and psychometric data were measured from 20 men professional athletes assisted by the SSD and 20 men living in the same violent community. The comparison of groups revealed greater sympathetic parameters of HRV, positive affect, and quality of life in the SSD group. Multiple regression analysis showed that the quality of life in the SSD group was positively predicted by positive affect, while in the control group the quality of life was negatively predicted by their history of traumatic events. Both groups reported high levels of exposure to traumatic events and posttraumatic stress symptoms. However, this study demonstrates the benefits of SSD programs in mental health.Item The different impacts of COVID-19 on the mental health of distinct health care worker categories.(2022) Machado, Arthur Viana; Gonçalves, Raquel Menezes; Gama, Camila Monteiro Fabricio; Vilete, Liliane Maria Pereira; Berger, William; Passos, Roberta Benitez Freitas; Mendlowicz, Mauro Vito; Souza, Gabriela Guerra Leal de; Pereira, Mirtes Garcia; Mocaiber, Izabela; Oliveira, Letícia deThe present study sought to explore the factors associated with the odds of having probable depression and posttraumatic stress disorder (PTSD) related to traumatic COVID-19 experiences and their impact on health care workers in distinct categories. In this cross-sectional study, 1843 health care workers (nurses, nurse technicians, physicians, physical therapists, and other healthcare workers) were recruited via convenience sampling. A survey was administered to obtain information regarding sociodemographic, occupational, and mental health status. Descriptive statistics and multivariable logistic regression were used for the analyses. Being a nurse technician was associated with an odds ratio of 1.76 for probable PTSD. No relation was observed between health care worker categories and the odds of probable depression. Additionally, being female and not receiving adequate PPE were related to greater odds of having probable PTSD and depression.Item Understanding the synergistic antioxidant mechanism of a layered double hydroxide–lycopene composite by spectroscopic techniques.(2023) González Rojas, Norma Alejandra; Moisés, Fermín Paul Pacheco; Cruz Ramos, José Alfonso; Bezerra, Frank Silva; Carbajal Arízaga, Gregorio GuadalupeLycopene is one of the most powerful natural antioxidants with demonstrated capacity to protect and reduce risks against some diseases. It has also been used as additive in polymers for packaging. Hydrophobicity is the main disadvantage of this compound, especially to prepare edible formulations and assimilation in the body; to improve this, emulsions have been formulated although they provide a low content of lycopene. For this study, we prepared a hybrid composite material with layered double hydroxides (LDHs) and 54% of lycopene. The composite is easily dispersed in water and when subjected to aqueous media to simulate a pass through stomach (pH 1.2), small intestine (pH 6.8) and large intestine (pH 7.4), where the composite released 84.5%, 92.8 and 96.7% of lycopene, respectively. The composite was analyzed in each step. The antioxidant capacity of the composite was similar to that of pure lycopene, although the content of lycopene was partial. Electron paramagnetic resonance (EPR) and X-ray photoelectron spectroscopy (XPS) indicated that the cations from LDH particles also contribute to capture unpaired electrons, thus producing a synergy in the antioxidant activity. Further, UV–vis and infrared spectroscopies demonstrated that the lycopene molecule was protected within along the pass through the three simulated media. This composite is an alternative vehicle to prepare lycopene supplements for oral consumption, maximizing the stability, and enhancing the release and therapeutic profle. This composite is also an alternative antioxidant additive for polymers or biofuels.Item High-flow nasal cannula compared with continuous positive airway pressure : a bench and physiological study.(2022) Vieira, Fernando; Bezerra, Frank Silva; Coudroy, Remi; Schreiber, Annia; Telias, Irene; Dubo, Sebastián; Cavalot, Giulia; Pereira, Sergio Martins; Piraino, Thomas; Brochard, Laurent JeanHigh-flow nasal cannula (HFNC) is extensively used for acute respiratory failure. However, questions remain regarding its physiological effects. We explored 1) whether HFNC produced similar effects to continuous positive airway pressure (CPAP); 2) possible explanations of respiratory rate changes; 3) the effects of mouth opening. Two studies were conducted: a bench study using a manikin’s head with lungs connected to a breathing simulator while delivering HFNC flow rates from 0 to 60 L/min; a physiological crossover study in 10 healthy volunteers receiving HFNC (20–60 L/min) with the mouth open or closed and CPAP 4 cmH2O delivered through face mask. Nasopharyngeal and esophageal pressures were measured; tidal volume and flow were estimated using calibrated electrical impedance tomography. In the bench study, nasopharyngeal pressure at end-expiration reached 4 cmH2O with HFNC at 60 L/min, whereas tidal volume decreased with increasing flow. In volunteers with HFNC at 60 L/min, nasopharyngeal pressure reached 6.8 cmH2O with mouth closed and 0.8 cmH2O with mouth open; P < 0.001. When increasing HFNC flow, respiratory rate decreased by lengthening expiratory time, tidal volume did not change, and effort decreased (pressure-time product of the respiratory muscles per minute); effort was equivalent between CPAP and HFNC 40 L/min and became lower at 60 L/min (P = 0.045). During HFNC with mouth closed, and not during CPAP, resistance to breathing was increased, mostly during expiration. In conclusion, mouth closure during HFNC induces a positive nasopharyngeal pressure proportional to flow rate and an increase in expiratory resistance that might explain the prolonged expiration and reduction in respiratory rate and effort, and contribute to physiological benefits. NEW & NOTEWORTHY High-flow nasal cannula generates positive airway pressure and reduces respiratory rate. Our findings confirm these physiological effects when the mouth is kept closed. An increased resistance to breathing induces a longer expiratory phase, which leads to decreased respiratory rate and minute ventilation, and therefore to a decreased respiratory workload, counterbalancing the increased pressure-time product per liter. These effects are less apparent with the mouth open and are not observed with continuous positive airway pressure.Item Netzahualcoyonol from Salacia multiflora (Lam.) DC. (Celastraceae) roots as a bioactive compound against gram-positive pathogens.(2022) Nizer, Waleska Stephanie da Cruz; Ferraz, Ariane Coelho; Moraes, Thaís de Fátima Silva; Lima, William Gustavo de; Santos, Josana Pereira dos; Duarte, Lucienir Pains; Magalhães, Cíntia Lopes de Brito; Vieira Filho, Sidney Augusto; Magalhães, José Carlos deThe expression of virulence factors, such as biofilm formation, in association with the acquisition of resistance to multiple drugs, has evidenced the need for new and effective antimicrobial agents against Staphylococcus aureus. The evaluation of the pharmacological properties of plant-derived compounds is a promising alternative to the development of new antimicrobials. In this study, we aimed to evaluate the antibacterial, antibiofilm, and the synergistic and cytotoxic effects of netzahualcoyonol isolated from Salacia multiflora (Lam.) DC. roots. Netzahualcoyonol presented bacteriostatic (1.56–25.0 mg/mL) and bactericidal (25.0–400.0 mg/mL) effects against Gram-positive bacteria, disrupted the biofilm of S. aureus, and presented a synergistic effect after its combination with b-lactams and aminoglycosides. The low cytotoxicity of netzahualcoyonol (Selectivity Index (SI) for S. aureus (2.56), S. saprophyticus (20.56), and Bacillus subtilis (1.28)) suggests a good security profile. Taken together, these results show that netzahualcoyonol is promising for the development of a new effective antibacterial agent.Item Lactobacillus delbrueckii UFV-H2b20 increases IFN-γ production and CD39+CD73+ Treg cell numbers in lungs, and protects mice against experimental allergic asthma.(2022) Andrade, Ana Clara Matoso Montuori de; Silva, Ana Elisa Nolasco e; Malacco, Nathalia Luisa Sousa de Oliveira; Vaz, Leonardo Gomes; Afonso, Luís Carlos Crocco; Russo, Remo de Castro; Vieira, Leda Quercia; Santos, Liliane Martins dosAsthma is a disorder characterized by airflow obstruction, inflammation, declining airway function, bronchial hyperresponsiveness and tissue remodelling. Probiotics are defined as “live microorganisms that, when administered in adequate amounts, confer a health benefit on the host”. The use of probiotics is becoming increasingly studied and recent evidence has suggested that it may provide therapeutic benefits in asthma and other diseases. Lactobacillus delbrueckii UFV-H2b20 fulfils all the requirements to be classified as probiotic. Previous studies have already shown the ability of L. delbrueckii UFV-H2b20 to stimulate the immune system. Our objective was to evaluate the protective effects of L. delbrueckii UFV-H2b20 in experimental allergic asthma. We used a murine model of ovalbumin-induced allergic airway inflammation to mimic allergic asthma. Oral treatment with L. delbrueckii UFV-H2b20 improves respiratory parameters and inhibits the inflammatory response in the lungs by decreasing the numbers of inflammatory monocytes, eosinophils and alveolar macrophages, as well as IgE levels. Treatment increased the IFN-γ/IL-4 cytokine ratio. Levels of IL-10 in the lungs were also increased in treated animals. Our results also showed that the probiotic administration increases the number of CD39+CD73+ T regulatory lymphocytes in the lung, suggesting a role for purinergic signals in the regulation of inflammation promoted by the treatment. Understanding the mechanisms of modulation of the immune system by probiotics could allow the development of probiotic preparations that are safe and have a direct action. Our results suggest that oral administration of L. delbrueckii UFV-H2b20 could be helpful to treat chronic inflammatory airway diseases, such as asthma.