Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.
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2022
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Background: In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of
the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less
complicated clinical and laboratory techniques.
Methods: In this retrospective study, NTM species isolated from pulmonary, extrapulmonary, and disseminated samples from patients
treated at a TB/HIV reference unit in the State of Amazonas from 2011 to 2014 were identified through a combination of molecular
techniques.
Results: To identify the molecular technique, 50 cryopreserved NTM cultures were recovered and subcultivated in culture medium. The
potentially pathogenic NTM species identified were M. avium, M. intracellulare, M. kansasii, M. chelonae, M. abscessus, M. fortuitum, and M.
peregrinum. Results of GenoType® showed moderate agreement with those of genomic sequencing (kappa = 0.60), whereas the results
obtained by the PRA-hsp65 technique disagreed with the results obtained by sequencing (kappa = 0.49).
Conclusions: Our findings highlight that GenoType CM is a good method for the identification of NTM, as well as the need for the
application of standardized criteria, such as those set forth by the ATS.
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HIV infections, Molecular diagnostic technique, Nontuberculous mycobacteria, Prevalence
Citação
LIMA, A. C. de O. de et al. Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil. Sociedade Brasileira de Medicina Tropical, v. 55, artigo e0613-2021, 2022. Disponível em: <https://www.scielo.br/j/rsbmt/a/WHpMgr9PXzxNwDDzT735sqJ/?lang=en>. Acesso em: 01 ago. 2023.