Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis.

dc.contributor.authorResende, Lucilene Aparecida
dc.contributor.authorRoatt, Bruno Mendes
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorViana, Kelvinson Fernandes
dc.contributor.authorMendonça, Ludmila Zanandreis de
dc.contributor.authorLanna, Mariana Ferreira
dc.contributor.authorLemos, Denise da Silveira
dc.contributor.authorOliveira, Rodrigo Corrêa de
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorFujiwara, Ricardo Toshio
dc.contributor.authorCarneiro, Cláudia Martins
dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorGiunchetti, Rodolfo Cordeiro
dc.date.accessioned2015-01-22T14:54:37Z
dc.date.available2015-01-22T14:54:37Z
dc.date.issued2013
dc.description.abstractIn the studies presented here, dogs were vaccinated against Leishmania (Leishmania) cha-gasi challenge infection using a preparation of Leishmania braziliensis promastigote proteinsand saponin as adjuvant (LBSap). Vaccination with LBSap induced a prominent type 1immune response that was characterized by increased levels of interleukin (IL-) 12 andinterferon gamma (IFN- _) production by peripheral blood mononuclear cells (PBMC) uponstimulation with soluble vaccine antigen. Importantly, results showed that this type ofresponsiveness was sustained after challenge infection; at day 90 and 885 after L. chagasichallenge infection, PBMCs from LBSap vaccinated dogs produced more IL-12, IFN- _ andconcomitant nitric oxide (NO) when stimulated with Leishmania antigens as comparedto PBMCs from respective control groups (saponin, LB- treated, or non-treated controldogs). Moreover, transforming growth factor (TGF)- _ decreased in the supernatant of SLcA-stimulated PBMCs in the LBSap group at 90 days. Bone marrow parasitological analysisrevealed decreased frequency of parasitism in the presence of vaccine antigen. It is con-cluded that vaccination of dogs with LBSap vaccine induced a long-lasting type 1 immuneresponse against L. chagasi challenge infection.pt_BR
dc.identifier.citationRESENDE, L. A. et al. Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis. Veterinary Parasitology, v. 198, p. 371-381, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401713005050>. Acesso em: 13 ago. 2013.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.vetpar.2013.09.011
dc.identifier.issn0304-4017
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4330
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3440300508466.pt_BR
dc.subjectVaccinept_BR
dc.subjectCanine visceral leishmaniasispt_BR
dc.subjectCytokinespt_BR
dc.subjectimmunoprotectionpt_BR
dc.subjectLeishmania chagasipt_BR
dc.titleCytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis.pt_BR
dc.typeArtigo publicado em periodicopt_BR

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
ARTIGO_CytokineNitricOxide.pdf
Tamanho:
573.88 KB
Formato:
Adobe Portable Document Format

Licença do pacote

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura Disponível
Nome:
license.txt
Tamanho:
2.57 KB
Formato:
Item-specific license agreed upon to submission
Descrição: