Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis.
dc.contributor.author | Resende, Lucilene Aparecida | |
dc.contributor.author | Roatt, Bruno Mendes | |
dc.contributor.author | Soares, Rodrigo Dian de Oliveira Aguiar | |
dc.contributor.author | Viana, Kelvinson Fernandes | |
dc.contributor.author | Mendonça, Ludmila Zanandreis de | |
dc.contributor.author | Lanna, Mariana Ferreira | |
dc.contributor.author | Lemos, Denise da Silveira | |
dc.contributor.author | Oliveira, Rodrigo Corrêa de | |
dc.contributor.author | Martins Filho, Olindo Assis | |
dc.contributor.author | Fujiwara, Ricardo Toshio | |
dc.contributor.author | Carneiro, Cláudia Martins | |
dc.contributor.author | Reis, Alexandre Barbosa | |
dc.contributor.author | Giunchetti, Rodolfo Cordeiro | |
dc.date.accessioned | 2015-01-22T14:54:37Z | |
dc.date.available | 2015-01-22T14:54:37Z | |
dc.date.issued | 2013 | |
dc.description.abstract | In the studies presented here, dogs were vaccinated against Leishmania (Leishmania) cha-gasi challenge infection using a preparation of Leishmania braziliensis promastigote proteinsand saponin as adjuvant (LBSap). Vaccination with LBSap induced a prominent type 1immune response that was characterized by increased levels of interleukin (IL-) 12 andinterferon gamma (IFN- _) production by peripheral blood mononuclear cells (PBMC) uponstimulation with soluble vaccine antigen. Importantly, results showed that this type ofresponsiveness was sustained after challenge infection; at day 90 and 885 after L. chagasichallenge infection, PBMCs from LBSap vaccinated dogs produced more IL-12, IFN- _ andconcomitant nitric oxide (NO) when stimulated with Leishmania antigens as comparedto PBMCs from respective control groups (saponin, LB- treated, or non-treated controldogs). Moreover, transforming growth factor (TGF)- _ decreased in the supernatant of SLcA-stimulated PBMCs in the LBSap group at 90 days. Bone marrow parasitological analysisrevealed decreased frequency of parasitism in the presence of vaccine antigen. It is con-cluded that vaccination of dogs with LBSap vaccine induced a long-lasting type 1 immuneresponse against L. chagasi challenge infection. | pt_BR |
dc.identifier.citation | RESENDE, L. A. et al. Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis. Veterinary Parasitology, v. 198, p. 371-381, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401713005050>. Acesso em: 13 ago. 2013. | pt_BR |
dc.identifier.doi | https://doi.org/10.1016/j.vetpar.2013.09.011 | |
dc.identifier.issn | 0304-4017 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/4330 | |
dc.language.iso | en_US | pt_BR |
dc.rights.license | O periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3440300508466. | pt_BR |
dc.subject | Vaccine | pt_BR |
dc.subject | Canine visceral leishmaniasis | pt_BR |
dc.subject | Cytokines | pt_BR |
dc.subject | immunoprotection | pt_BR |
dc.subject | Leishmania chagasi | pt_BR |
dc.title | Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |