Effects in vitro and in vivo of hesperidin administration in an experimental model of acute lung inflammation.
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2022
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Mechanical ventilation (MV) is a tool used in critical patient care. However, it can trigger inflammatory and
oxidative processes capable of causing or aggravating lung injuries, which is known as ventilator-induced lung
injury (VILI). Hesperidin is a flavonoid with antioxidant and anti-inflammatory properties in various diseases.
The role of hesperidin in the process triggered by MV is poorly studied. Thus, we hypothesize hesperidin could
protect the lung of mice submitted to mechanical ventilation. For that, we evaluated cell viability and reactive
oxygen species (ROS) formation in macrophages using different hesperidin concentrations. We observed hes-
peridin did not reduce cell viability, however; it attenuated the production of intracellular ROS in cells stimu-
lated with lipopolysaccharide (LPS). We further evaluated the effects of hesperidin in vivo in animals submitted
to MV. In the bronchoalveolar lavage fluid, there were higher levels of macrophage, lymphocyte and neutrophil
counts in animals submitted to MV, indicating an inflammatory process. In the lung tissue, MV induced oxidative
damage and increased myeloperoxidase activity, though the antioxidant enzyme activity decreased. MV also
induced the production of the inflammatory mediators CCL-2, TNF-α and IL-12. Pretreatment with hesperidin
resulted in less recruitment of inflammatory cells to the airways and less oxidative damage. Also, it reduced the
formation of CCL-2 and IL-12. Our results show pretreatment with hesperidin can protect the lungs of mice
submitted to mechanical ventilation by modulating the inflammatory response and redox imbalance and may act
to prevent MV injury.
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Antioxidants, Inflammation, Hesperidin, Mechanical ventilation, Redox imbalance
Citação
SOUZA, A. B. F. de et al. Effects in vitro and in vivo of hesperidin administration in an experimental
model of acute lung inflammation. Antioxidants, v. 180, p. 253-262, fev. 2022. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0891584922000387?via%3Dihub>. Acesso em: 11 out. 2022.