A new extraction and purification methodology of Bowman-Birk inhibitors from seeds and germinated seeds of Macrotyloma axillare.

Abstract

The Macrotyloma axillare Bowman-Birk inhibitors from seeds and germinated seeds (120 h) were purified by an alternative methodology and revealed the presence of different contents of inhibitors for each treatment. Mass analyses of inhibitors, from seeds and germinated seeds, showed masses of about 7 and 8 kDa. The 120 h-germinated seed fractions presented higher activity isoforms than seeds without treatment.