Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.

dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorCarvalho, Andréa Teixeira de
dc.contributor.authorGiunchetti, Rodolfo Cordeiro
dc.contributor.authorGuerra, Luanda Liboreiro
dc.contributor.authorCarvalho, Maria das Graças
dc.contributor.authorMayrink, Wilson
dc.contributor.authorGenaro, Odair
dc.contributor.authorOliveira, Rodrigo Corrêa de
dc.contributor.authorMartins Filho, Olindo Assis
dc.date.accessioned2017-02-20T12:30:34Z
dc.date.available2017-02-20T12:30:34Z
dc.date.issued2006
dc.description.abstractCanine visceral leishmaniasis (CVL) manifests itself as a broad clinical spectrum ranging from asymptomatic infection to patent severe disease. Despite relevant findings suggesting changes on lymphocytes subsets regarding the CVL clinical forms, it still remains to be elucidated whether a distinct phenotypic profile would be correlated with degree of tissue parasite density.Herein, we have assessed the correlation between the clinical status as well as the impact of bone marrow parasite density on the phenotypic profile of peripheral blood leucocytes in 40 Brazilian dogs naturally infected by Leishmania chagasi. Our major findings describe the lower frequency of B cells and monocytes as the most important markers of severe CVL. Our main statistically significant findings reveal that the CD8+ T cell subset reflects most accurately both the clinical status and the overall bone marrow parasite density, as increased levels of CD8+ lymphocytes appeared as the major phenotypic feature of asymptomatic disease and dogs bearing a low parasite load. Moreover, enhanced major histocompatibility complex (MHC)-II density as well as a higher CD45RB/CD45RA expression index seems to represent a key element to control disease morbidity. The association between clinical status, bone marrow parasitism and CD8+ T cells re-emphasizes the role of the T cellmediated immune response in the resistance mechanisms during ongoing CVL. Higher levels of circulating T lymphocytes (both CD4+ and CD8+ T cells) and lower MHC-II expression by peripheral blood lymphocytes seem to be the key for the effective immunological response, a hallmark of asymptomatic CVL.pt_BR
dc.identifier.citationREIS, A. B. et al. Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi. Clinical And Experimental Immunology, v. 146, p.303-311, 2006. Disponível em: <https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2249.2006.03206.x>. Acesso em: 10 out. 2016.
dc.identifier.doihttps://doi.org/10.1111/j.1365-2249.2006.03206.x
dc.identifier.issn1365-2249
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/7273
dc.identifier.uri2https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2249.2006.03206.xpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectBone marrow parasitismpt_BR
dc.subjectCanine visceralpt_BR
dc.subjectClinical statuspt_BR
dc.titlePhenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.pt_BR

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