Liver infusion tryptose (LIT) : the best choice for growth, viability, and infectivity of Leishmania infantum parasites.

dc.contributor.authorCosta, Ana Flávia Pereira
dc.contributor.authorBrito, Rory Cristiane Fortes de
dc.contributor.authorCarvalho, Lívia Mendes
dc.contributor.authorCardoso, Jamille Mirelle de Oliveira
dc.contributor.authorVieira, Paula Melo de Abreu
dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorRoatt, Bruno Mendes
dc.date.accessioned2021-09-03T14:14:51Z
dc.date.available2021-09-03T14:14:51Z
dc.date.issued2020pt_BR
dc.description.abstractLeishmania spp. parasites have a complex biological cycle presenting basically two different morphological stages, the amastigote and promastigote forms. In vitro cultivation allows a more complete study of the biological aspects of these parasites, indicating better conditions for infection, immunoassay tests, drug evaluations, and vaccines. Thus, we evaluated the three most used culture media for Leishmania spp., Grace’s insect cell culture medium (Grace’s), liver infusion tryptose (LIT), and Schneider’s insect medium (Schneider’s), without supplementation or supplemented with fetal calf serum (FCS) and bovine serum albumin (Albumin) to evaluate the growth, viability, and infectivity of the L. infantum promastigotes. It was observed that promastigote forms have a better growth in LIT and Schneider’s with or without FCS when compared to that in Grace’s. The supplementation with albumin promoted greater viability of the parasites independent of the medium. For in vitro infection of J774.A1 macrophages using light microscopy and flow cytometry analyses, FCS-supplemented LIT and Grace’s promoted higher percentage of infected macrophages and parasite load compared with Schneider’s media. Taken together, our results demonstrated that the supplementation of LIT culture medium with FCS is the most suitable strategy to cultivate Leishmania infantum parasites enabling the maintenance of growth and infective parasites for research uses.pt_BR
dc.identifier.citationCOSTA, A. F. P. et al. Liver infusion tryptose (LIT): the best choice for growth, viability, and infectivity of Leishmania infantum parasites. Parasitology Research, v. 119, p. 4185-4195 , 2020. Disponível em: <https://link.springer.com/article/10.1007/s00436-020-06893-z>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00436-020-06893-zpt_BR
dc.identifier.issn1432-1955
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/13640
dc.identifier.uri2https://link.springer.com/article/10.1007/s00436-020-06893-zpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectIn vitro cultivationpt_BR
dc.subjectGrace’s insect cell culture mediumpt_BR
dc.subjectSchneider’s insect mediumpt_BR
dc.titleLiver infusion tryptose (LIT) : the best choice for growth, viability, and infectivity of Leishmania infantum parasites.pt_BR
dc.typeArtigo publicado em periodicopt_BR

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